CD8 anticorps (C-Term, Cytoplasmic Domain)

N° du produit ABIN3024487

Cet anticorps anti-CD8 est un anticorps Souris Monoclonal détectant CD8 dans FACS, IF et IHC (p). Adapté pour Humain.

Aperçu rapide pour CD8 anticorps (C-Term, Cytoplasmic Domain) (ABIN3024487)

Antigène: CD8

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: C8-144B

Détail du produit anti-CD8 anticorps

Épitope: C-Term, Cytoplasmic Domain

Purification: Protein G affinity chromatography

Immunogène: A 13 amino acid peptide from the C-terminal cytoplasmic domain of the alpha chain was used as immunogen for this CD8 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD8 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells,Immunofluorescence: 0.5-1 μg/mL,Immunohistology (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2),

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: CD8 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur CD8

Antigène: CD8

Autre désignation: CD8

Sujet: CD8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD8 and 4 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8a antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral Tcell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+/CD8-, and in T-lymphoblastic lymphoma/leukemia, they are often co-expressed. It is also found in littoral cell angioma of the spleen.